বৃহস্পতিবার, ২৪ জানুয়ারী, ২০১৩

DNA 'perfect for digital storage'

Scientists have given another eloquent demonstration of how DNA could be used to archive digital data.

The UK team encoded a scholarly paper, a photo, Shakespeare's sonnets and a portion of Martin Luther King's I Have A Dream speech in artificially produced segments of the "life molecule".

The information was then read back out with 100% accuracy.

It is possible to store huge volumes of data in DNA for thousands of years, the researchers write in Nature magazine.

They acknowledge that the costs involved in synthesizing the molecule in the lab make this type of information storage "breathtakingly expensive" at the moment, but argue that newer, faster technologies will soon make it much more affordable, especially for long-term archiving.

"One of the great properties of DNA is that you don't need any electricity to store it," explained team-member Dr Ewan Birney from the European Bioinformatics Institute (EBI) at Hinxton, near Cambridge.

"If you keep it cold, dry and dark - DNA lasts for a very long time. We know that because we routinely sequence woolly mammoth DNA that is kept by chance in those sorts of conditions." Mammoth remains are many thousands of years old.

The group cites government and historical records as examples of data that could benefit from the molecular storage option.

Much of this information is not required every day but still needs to be kept. Once encoded in DNA, it could be put away safely in a vault until it was needed.

And unlike other storage media presently in use such as hard disk-drives and magnetic tapes, the DNA "library" would not demand constant maintenance.

In addition, the universality of the life molecule means there would probably never be a backwards-compatibility issue where the technology of the day was incapable of reading the vault's archives.

"We think there will always be DNA-reading technology so long as there is DNA-based life around on Earth, assuming it is technologically sophisticated of course," Dr Birney told BBC News.

This is not the first time that DNA has been used to encode the sort of routine information we keep on our computers.

Last year, for example, an American group published the results of a very similar experiment in Science Magazine. The Boston researchers laid down a whole book in DNA.

The EBI study uses slightly different techniques to achieve its goals, but has also looked deeper into some of the issues of scalability and practicality.

Underpinning all these approaches is the exploitation of the nucleobase sequence at the heart of DNA.

The helical molecule is famously held together by four chemical groups, or nucleobases, which, when arranged in a specific order, carry the genetic instructions needed by a living organism to build and maintain itself.

The EBI storage system uses the same four "letters" but in a completely different "language" to the one understood by life.

To copy a computer file, such as a text document, the binary digits (zeros and ones) that would ordinarily represent that information on a hard drive first have to be translated into the team's bespoke code. A standard DNA synthesis machine then churns out the corresponding sequence.

But it is not one long molecule. Rather, it is multiple copies of overlapping fragments, with each fragment also carrying some indexing details that identify where in the overall sequence it should sit.

This builds redundancy into the system, meaning that if some fragments become corrupted, the data will not be lost.

Again, the same standard equipment used in molecular biology labs to read the DNA of organisms is used to pull out the information so that it can be displayed on a computer screen once more.

For its experiment, the EBI team encoded a 26-second snippet of Martin Luther King's classic anti-racism address from 1963, a ".jpg" photo of the EBI (see right); a ".pdf" of the seminal 1953 paper by Crick and Watson describing the structure of DNA, ".txt" file containing all of Shakespeare's sonnets; and a file about the encoding system itself (a total equivalent on a computer drive to about 760 kilobytes).

Physically, the DNA carrying all that information is no bigger than a speck of dust.

Team member Nick Goldman said the molecule was an incredibly dense storage medium. One gram of DNA ought to be able to hold about two petabytes of data, he added - the equivalent of about three million CDs.

Dr Goldman addressed the concern some people might have that artificial DNA code could somehow go wild and end up in the genome of another living organism.

"The DNA we've created can't be incorporated accidently into a genome; it uses a completely different code to what the cells of living bodies use," he explained.

"And if you did end up with any of this DNA inside you, it would just be degraded and disposed of. It really has no place in a living being."

Jonathan.Amos-INTERNET@bbc.co.uk and follow me on Twitter: @BBCAmos

Source: http://www.bbc.co.uk/news/science-environment-21145163#sa-ns_mchannel=rss&ns_source=PublicRSS20-sa

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Ireland won't ease drunken-driving law for farmers

DUBLIN (AP) ? A license to drive drunk? Some small-town politicians think it's just the tonic for rural Ireland.

Councilmen in Kerry, southwest Ireland, passed a motion this week asking the government to create a permit that would allow isolated farmers the ability to drink a few pints and then return home in their car, or on their tractor, without fear of being busted.

Its backers say the measure is needed to combat an epidemic of boredom and depression on farms ever since Ireland imposed tough new blood-alcohol limits on drivers in 2011.

But Justice Minister Alan Shatter shot down the proposal during a speech in parliament Thursday as "grossly irresponsible."

"There is no question of this government, or indeed I don't believe any future government, facilitating individuals drinking in excess of the blood alcohol limits," Shatter told lawmakers.

A generation ago, drunken driving was commonplace in Ireland and even the smallest villages or forlorn crossroads would feature a pub. But in this century the country has steadily improved road safety standards, introducing mandatory driving tests, blood and breath tests and above all a penalty-points system that removes licenses from dangerous drivers, particularly drunks.

The effort has slashed road-related deaths from more than 400 annually in the 1990s to just 162 last year, a modern low in this country of 4.6 million.

Kerry pub owners say their business has plummeted right along with that nationwide carnage ? yet deny any connection between the two trends. They describe the often narrow, lightly trafficked roads near their businesses as safe for people to navigate even after three pints (57 ounces) of beer.

Danny Healy-Rae, who owns a pub and comes from Kerry's most famous and flamboyant political family, says farmers should be allowed to drive tipsy on their tractors because they don't go fast enough to kill anyone. He said those drinking two to three pints at a pub should be issued a permit allowing them to drive home so long as they stay below 30 mph (50 kph).

He was one of five Kerry County Council members who voted for the motion Monday night. Three others voted against, seven abstained and 12 council members didn't show up. Their decision has no legal standing because the national government, not councils, sets policy on road safety.

Healy-Rae ? who like his politician father is nationally famous for wearing a cap everywhere and talking in rapid-fire local dialect easy to parody but hard to understand ? said pub-loving farmers "are living in isolated rural areas where there's no public transport of any kind. They end up at home looking at the four walls, night in and night out, because they don't want to take the risk of losing their license."

He said the older generation provided the sociological fuel to Ireland's tradition of pub-based music and "craic," Irish slang for entertaining conversation.

"All the wisdom and all the wit and all the culture that they had, the music and the singing, that's all being lost to the younger generation," Healy-Rae said. "These older people might as well be living in Japan and Jerusalem, because the younger generation don't see them at all anymore."

Yet even in Kerry, many have dismissed the idea as both dangerous to public safety and impossible to enforce. And alcohol-abuse campaigners say Healy-Rae's logic is twisted, since alcohol is a depressant and hardly a cure for the blues.

"The link between alcohol use and suicide has been well established and drinking alcohol will exacerbate, not alleviate, any mental health difficulties that a person may be struggling with, such as depression or anxiety," said Conor Cullen, spokesman for a pressure group called Alcohol Action Ireland.

The government emphasized that Kerry's council motion was already a dead letter.

"While rural isolation is a real problem," said Transport Minister Leo Varadkar, "the solution to it is not to hand out drink-driving permits."

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/3d281c11a96b4ad082fe88aa0db04305/Article_2013-01-24-Ireland-Drunken%20Driving/id-77521f622af942fc8ccb347951423a92

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Apple Shares Plunge 10 Percent After Hours On Earnings Decline And Revenue Miss

Apple Shares Plunge 10 Percent After Hours On Earnings Decline And Revenue Miss
The law of large numbers kicked in Wednesday for Apple, which saw overall revenue just missing analyst estimates, despite the company racking up a record $13.1 billion in profits.

Source: http://feedproxy.google.com/~r/GearFactor/~3/TYLzeNDyWmQ/

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বুধবার, ২৩ জানুয়ারী, ২০১৩

Teenagers avoid early alcohol misuse through personality management

Jan. 23, 2013 ? Personality-targeted interventions delivered by trained teachers and school staff decrease alcohol misuse in at-risk teens and delay their classmates' alcohol uptake.

In a study published in the very first issue of the new journal JAMA Psychiatry, researchers from Sainte-Justine University Hospital Center, University of Montreal and King's College London have shown that personality-targeted school interventions delivered to high risk adolescents manage to reduce and postpone problem drinking, which is responsible for 9% of the deaths in young people between the ages of 15 and 29 in developed countries. Furthermore, by delaying alcohol uptake in at-risk youth, low-risk youth apparently gain group immunity due to reduced drinking within their social network.

"Two factors determine problem drinking: personality and peer pressure," explains Dr. Patricia Conrod, the study's first author, who supports the assumption that approaching at-risk youth from the angle of mental health rather than information on the dangers of alcohol is more effective at preventing early-onset alcohol misuse. "Teaching young people how to better manage their personality traits or vulnerabilities helps them make the right decisions in given situations," she explained, "whether it is a matter of overcoming their fears, managing thoughts that make them very emotional, controlling their compulsions, analyzing objectively the intentions of others or improving their self-perception."

The researcher conducted a two-year study called Adventure in which high-school school staff in London, England, were trained to intervene with their ninth grade students (median age of 13.7 years) in order to determine whether their intervention could reduce or postpone the participants' misuse of alcohol in the long term. The development of consumption patterns was measured by observing the drinking rates, problem drinking rates, binge drinking rates, and growth in binge-drinking rates. In addition, the study also attempted to examine a possible group ("herd") immunity effect associated with these interventions.

Participating schools were divided into two groups (control and intervention). The "intervention" school group conducted the intervention at the beginning of the study, whereas the "control" school group benefited from the program and training later on. Out of the total sample, 1,210 students were identified as being more at risk of developing future alcohol dependence after having filled out a personality questionnaire. They presented at least one of the following personality profiles: "anxiety-sensitivity," "hopelessness," "impulsivity" or "sensation-seeking." Trained school facilitators met these students during two brief personalized group sessions and asked them, based on their personality profile, to describe their reactions to various real-life scenarios and try to react to them in a different way.

"The advantage of this approach is that it is easy to implement as opposed to community-based approaches. Teachers can be trained to implement the program and closely adhere to its objectives very effectively, especially since only two interventions are required," explained Dr. Conrod.

A similar study is now under way in 32 high schools in Montreal, Canada. The study is also adding a component that analyses youth styles of thinking. Schools interested in taking part in the program can visit the project's website at:

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Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/living_well/~3/Hit4_-t2nB8/130123164917.htm

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We live only to Protect

We live only to Protect

Six Elements are called to Protect 'The Immortal one' from the Darkness.

Owner:

Game Masters:

This topic is an Out Of Character part of the roleplay, ?We live only to Protect?. Anything posted here will also show up there.

Topic Tags:

Forum for completely Out of Character (OOC) discussion, based around whatever is happening In Character (IC). Discuss plans, storylines, and events; Recruit for your roleplaying game, or find a GM for your playergroup.
This is the auto-generated OOC topic for the roleplay "We live only to Protect"

You may edit this first post as you see fit.

User avatar
Polka
Member for 4 years



May I reserve the Immortal? :)

~^.'.^~Beautiful people come in different sizes~^.'.^~

User avatar
Kura Ravengade
Member for 1 years


yeah. you sure can :)

reserve for this is 24 hourse , is that ok?

User avatar
Polka
Member for 4 years


Can I reserve Air or Ice? You choose, I can't. XD

ETA: I lied, can I have Ice and Light? :D

Last edited by bandgeek on Tue Jan 22, 2013 4:42 pm, edited 1 time in total.

"She offered herself to the big, bad wolf and didn't scream when he took the first bite."

User avatar
bandgeek
Member for 1 years


Polka: yes that's okay. Ad do you prefer anime or real pictures for the FC :)

User avatar
Kura Ravengade
Member for 1 years


Do you mind me having air?

I have a thousand years of experience, went through a thousand years of battles, and have a thousand years of wisdom. What makes you think you'd out-smart me?

WHERE IS THE END OF THE WORLD NOW, HUH APOCOLYPSE THEORISTS?! Yeah, I said it. Hate me. I dare you! I DOUBLE DOG DARE YOU- -Sorry, but we are experiencing technical dificulties-
User avatar
Asher MstrImmortalis
Member for 1 years


Can i reserve water?

It's official, my reign of terror is coming to an end. Vega, rest in peace, because I will be doing the same...

User avatar
deathrisesagain
Member for 3 years


Kura- Which ever you prefer

BandGeek- can you choose one and I shall reserve it

Asher- you can have air :)

Death- you can have water

again it's a 24 hour reserve :) xxx

User avatar
Polka
Member for 4 years


One question, what's your age range for the elementalists?

User avatar
deathrisesagain
Member for 3 years


I don't mind, its born into the same family as the previous elementals

so whichever age you see fit :)

I'm going to do younger for my character

User avatar
Polka
Member for 4 years


Eep, never mind, I can't choose. Good luck.

User avatar
bandgeek
Member for 1 years


Hmm. Maybe I should go for Ice. This seemed nice, considering the fact that this is another element role-play. Does this have any more genre we should look out for that you didn't tagged?

User avatar
code6435
Member for 1 years


I will reserve ice for you

And a bit of action, maybe romance (though I'm not overly keen)
:)

User avatar
Polka
Member for 4 years




Ok, so i just thought of another question. What are the weapon limits? Like Medeval style, modern, or futuristic? I'm asking for those who may have a character that rather use weapons then their abilities.

User avatar
deathrisesagain
Member for 3 years


Well this is set in modern day.... I haven't created a roleplay for such a long time I'm a bit rusty so sorry you have to keep asking questions

SO modern weapons :)

User avatar
Polka
Member for 4 years


It's ok Polka, it's hard for the old folks like you and i to create a rp and have everything in it. Being around for so long, you tend to forget little things like this. It's kool Polka.
Would throwing knives count as modern?

User avatar
deathrisesagain
Member for 3 years


Yeah I don't see why not :)

By the way Death, you going for anime or real pic for you character ? that is something else I forgot to specify

User avatar
Polka
Member for 4 years


Probably real, i tend to stay away from anime, because i could never tell if they are female or male, or their ages and such.

User avatar
deathrisesagain
Member for 3 years



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মঙ্গলবার, ২২ জানুয়ারী, ২০১৩

Enzyme helps cancer cells avoid genetic instability

Jan. 21, 2013 ? Cancer cells are resourceful survivors with plenty of tricks for staying alive. Researchers have uncovered one of these stratagems, showing how cells lacking the tumor suppressor BRCA1 can resume one form of DNA repair, sparing themselves from stagnation or death. The study appears in the January 21st issue of The Journal of Cell Biology.

The BRCA1 protein helps to mend double-strand DNA breaks by promoting homologous recombination. Without it, cells can amass broken, jumbled, and fused chromosomes, which may cause them to stop growing or die. Although cells lacking BRCA1 seem like they should be vulnerable, loss of the protein instead seems to boost abnormal growth.

Recent studies have shown that cells lacking BRCA1 compensate by cutting back on 53BP1. This protein helps orchestrate a different DNA repair mechanism, nonhomologous end joining (NHEJ), and it thwarts a key step in homologous recombination. Researchers think that, in cells without BRCA1, 53BP1 spurs excessive NHEJ that can cause fatal chromosomal chaos. But with 53BP1 out of the way, the cells are able to resume homologous recombination. That might explain why cells that lack BRCA1 and eliminate 53BP1 can withstand traditional chemotherapy compounds and PARP inhibitors, a new generation of anti-cancer drugs that are in clinical trials. But how do cancer cells turn down 53BP1?

Researchers previously found that certain mutant fibroblasts increase production of cathepsin L, a protease that destroys 53BP1. BRCA1-deficient cancer cells take advantage of the same mechanism, according to a team of researchers led by Susana Gonzalo from the Washington University School of Medicine. When they cultured breast cancer cells that were missing BRCA1, the cells stopped growing. After two weeks of lethargy, however, some cells, which the researchers dubbed BOGA cells (BRCA1-deficient cells that overcome growth arrest), began to divide again. These cells showed increased levels of cathepsin L and reduced amounts of 53BP1. Eliminating cathepsin L from BOGA cells or dosing them with vitamin D, a cathepsin L inhibitor, prevented the decline in 53BP1 abundance.

To find out whether boosting cathepsin L levels enabled the cancer cells to restart homologous recombination, the researchers monitored sites of DNA damage tagged by RAD51, a protein that helps promote homologous recombination. The cells that had stopped growing did not display RAD51 foci, but these foci were prevalent in BOGA cells with reduced 53BP1. Removing cathepsin L from BOGA cells increased 53BP1 levels and diminished the number of RAD51 foci.

If cells can't perform homologous recombination, they turn to repair mechanisms such as NHEJ that can lead to jumbled chromosomes. However, after DNA-breaking doses of radiation, BOGA cells exhibited few chromosome defects. The number of these flaws climbed after the researchers stabilized 53BP1 levels by inhibiting cathepsin L or trimming its abundance.

The team then analyzed tumor samples from breast cancer patients. Researchers suspect that cathepsin L attacks 53BP1 by entering the nucleus. Samples from patients with BRCA1 mutations or with triple-negative breast cancer -- an aggressive form of the disease -- showed high levels of nuclear cathepsin L and reduced quantities of 53BP1. That suggests tumors in these patients hike the amounts of cathepsin L in the nucleus to break down 53BP1 and restore homologous recombination.

"It's a new pathway that explains how breast cancer cells lose 53BP1," says Gonzalo. How cancer cells boost nuclear cathepsin L levels is unclear, she notes.

Triple-negative breast cancers are currently identified by their lack of Her2 and the estrogen and progesterone receptors. The work suggests that another trio of measurements -- the amounts of 53BP1, cathepsin L, and vitamin D receptor in the nucleus -- might help identify patients that are resistant to current breast cancer treatments. These people might respond to cathepsin inhibitors, some of which are undergoing animal testing. These compounds might steer the cells away from homologous recombination and leave them vulnerable to other therapies.

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The above story is reprinted from materials provided by Rockefeller University Press, via EurekAlert!, a service of AAAS.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. D. A. Grotsky, I. Gonzalez-Suarez, A. Novell, M. A. Neumann, S. C. Yaddanapudi, M. Croke, M. Martinez-Alonso, A. B. Redwood, S. Ortega-Martinez, Z. Feng, E. Lerma, T. Ramon y Cajal, J. Zhang, X. Matias-Guiu, A. Dusso, S. Gonzalo. BRCA1 loss activates cathepsin L-mediated degradation of 53BP1 in breast cancer cells. The Journal of Cell Biology, 2013; 200 (2): 187 DOI: 10.1083/jcb.201204053

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/top_health/~3/yzxjZotL_ng/130121122940.htm

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Lindsay Lohan Turns Down Dancing With the Stars, is Not Smart

Source: http://www.thehollywoodgossip.com/2013/01/lindsay-lohan-turns-down-dancing-with-the-stars-is-not-smart/

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Verizon Business Security Blog ? Blog Archive ? Official v1 JSON ...

Wade Baker
January 21st, 2013

Those of you familiar with the Vocabulary for Event Recording and Incident Sharing (VERIS) framework might have reviewed or experimented with the beta schema (XML) we released last year. Since then, we continued to test and refine the schema (thanks to those in the VERIS Community who provided input), and we?re happy to say that a full version 1 is now ready for prime time.

What does that mean?

Well, for those of you trying to implement VERIS into the processes and applications of your organization, it means stability. We released an initial version of VERIS nearly three years ago, but it has continued to be tweaked (you might argue ?overhauled?) since that time. This was a natural and necessary process as VERIS went from a set of data points used only by Verizon for a specific purpose (produce the DBIR) to a decently-documented and structured schema that has proven itself suitable to the end for which it was created ? sharing incident data.

And we hope you?ll use it to that end and that your organization will benefit from it in many ways.

If you?re interested in finding out more about VERIS, visit www.veriscommunity.net, which?has quite a bit of supporting information and resources. The schema itself can be obtained from GitHub (there is a main schema file (verisc.json) and one that includes the many enumerations specified within VERIS (verisc-enum.json).

If you have any questions about VERIS or its use, we encourage you to join the community mailing list and ask them.

Tags: DBIR, DFIR, Incident Response, information sharing, VERIS

Source: http://securityblog.verizonbusiness.com/2013/01/21/official-v1-json-schema-released-for-veris/

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সোমবার, ২১ জানুয়ারী, ২০১৩

Writing Resolutions (and some thoughts on white noise) ? the Well ...

Rabbit - lookIt?s the beginning of another year and I?ve been thinking a lot about my writing. 2012 was my first year as a full time writer with a book out (and then two). It was an amazing year but it certainly brought a bunch of new challenges, pressures, and the need to juggle more balls. My time has changed. It?s not that I don?t have time, it?s that time is precious and I want to use it wisely.

With that in mind I have three writing resolutions for 2013:

Enjoy Blogging Again

I began blogging because words were just busting out of me and I needed somewhere to put them. Plus I loved thinking about writing / illustration, and if I didn?t put my thoughts somewhere I was going to drive my family crazy speaking about it constantly. This hasn?t changed ? I?m still overflowing with words and love speaking about the industry (and still drive my family nuts). BUT nowadays I funnel most of my words into my manuscripts (deadlines!) and spend much time speaking about craft at festivals and schools. Weeks go by without me blogging and I feel guilty about it. And a guilty blogger is not a happy blogger. So: I?ve given myself permission to blog only when I feel like it, and about whatever writing stuff I want (rather than what I think I should blog about).

This means there might be spans of time where I don?t blog. If you want to keep up with me I?d recommend you:

Reduce White Noise

A big part of writing for me ? in fact, probably the biggest ? is the thinking. And I need lots of time to think: time where my mind wanders around and about and back again, allowing ideas to swirl and come together. I?ve been practising this skill my whole life. I started daydreaming as a kid (my grade 3 teacher wrote it on my report card as a negative but I knew otherwise). I think deeply about things. I?ve never been able to hear a story or anecdote without taking it a hundred steps further in my mind. And as a writer I rely on this trait.

That?s where white noise comes in. In the last few years we?ve gotten pretty good at eliminating ?wasted time? with the invention of the smart phone. We no longer sit on buses and stare out the window, or wait for a friend to arrive at a cafe by watching people go by. Because we pull out our phone. We?ve filled our heads with white noise ? Facebook and twitter and blogs and always being accessible by email. For most this may not be an issue. But for me I?ve removed my mind?s chance to wander, daydream and ask ?what if??. I?ve filled it with other people?s status updates, blog posts and (often) inane chatter. Why this is a problem: I don?t think about my stories as much. I have less new ideas.

My plan is to cut back the white noise. Severely. It?s not easy, as my iPhone habits are quite ingrained, but it?s getting easier. And guess what? The ideas are already flowing better.?I also like myself better when I spend less time on my phone. I smile more and have more to say about the world.

PS. My thoughts on this became more concrete after reading this awesome blog post on Nathan Bransford?s site (make sure you read right through).

Year of Writing

People assume that being a full time writer mostly involves a lot of ? well ? writing. Sadly, this isn?t so, which I discovered all too well this year. Most of my time has been consumed by business type stuff, promotion, travel and speaking work. This year has been a real eye opener. I?m still learning how to manage it all, and to be honest I?ve hardly created any new work. I miss writing. So I?m officially naming this my Year of Writing. I?ve resolved to put it first again.

Come hell, high water or the zombie apocalypse, there will be writing.

And on that note, I?m sneaking back to the first draft of my YA novel. 25,000 words and counting?

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Source: http://wellreadrabbit.wordpress.com/2013/01/22/writing-resolutions-and-some-thoughts-on-white-noise/

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Review: Rust and Bone | Film Reviews | Gambit New Orleans News ...

"I'm hungry," are the first words spoken in French director Jacques Audiard's tough-as-nails love story Rust and Bone. The line comes from a little boy named Sam whose apparently homeless father Ali (Matthias Schoenaerts) proceeds to feed him from scraps he finds on a passenger train. It's a fitting start for a film that chooses personal tragedy as its real starting point and goes on to depict the modern world as a relentlessly harsh and pitiless place. Everyone's hungry in Rust and Bone, but it's only through raw physicality that any of the movie's characters find meaning and rise above pain and emptiness.

??On paper, Rust and Bone is the sort of melodrama few people find appealing. It stars Marion Cotillard, the immensely talented Oscar-winning French actress (for La Vie En Rose) who recently expanded her American audience with key roles in Inception and The Dark Knight Rises. She plays Stephanie, an unhappy orca trainer at a cheesy marine park who loses her legs below the knees in a freak accident. Former boxer Ali becomes a nightclub bouncer, then begins fighting in dangerous bare-knuckle bouts designed to separate violence-hungry gamblers from their cash. His straightforward, almost childlike view of the world keeps him free of emotional entanglements and provides Stephanie with the perspective she needs to overcome post-accident depression. Will these two damaged souls wind up together? Is that a movie anyone really wants to see?

??Audiard manages to make Rust and Bone interesting, first by refusing to indulge in the sentiment that lesser directors would extract from such a story. The film is set in the idyllic and sun-drenched south of France, but Audiard reveals the same strip malls, big-box stores and tourist traps that blight similar stateside locales. Stephanie and Ali inhabit a working-class world where happy endings are few. (Ali moonlights as an installer of hidden cameras used to spy on employees and bust unions.) The visual style is stark but impressionistic. When Stephanie emerges back into a largely indifferent world, the beaches are lush, but the light is harsh and glaring.

??Both Cotillard and Schoenaerts deliver emotionally dry performances that support Audiard's organic realism. They give their characters' epic struggles the weight of authenticity, and the intimacy they build feels earned. Remarkably, Cotillard's especially moving turn was abetted by computer-generated imagery ? the illusion of her physical loss was created digitally in post-production, leaving her free to focus on her character's inner life. The results are completely convincing. Rust and Bone isn't always easy to watch, but Cotillard makes the tradeoff worthwhile. ? KEN KORMAN

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